PCOM Library / Hot Topics in Research / Archive for "Biomedical Sciences"

Category: Biomedical Sciences

Hot Topics: New Technique “Hotwires” Cells to Induce Endocytosis on Demand

Jackie Werner Biomedical Sciences, Hot Topics in Research

New tools for “hot-wiring” clathrin-mediated endocytosis with temporal and spatial precision Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ. New tools for “hot-wiring” clathrin-mediated endocytosis with temporal and spatial precision. J Cell Biol. 2017. http://dx.doi.org/10.1083/jcb.201702188. Clathrin-mediated endocytosis (CME) is the major route of receptor internalization at the plasma membrane. Analysis of constitutive CME is difficult because the initiation of endocytic events is unpredictable. When and where a clathrin-coated pit will form and what cargo it will contain are difficult to foresee. Here we describe a series of genetically encoded reporters that allow the initiation of CME on demand. A clathrin-binding…

Hot Topics: Transcriptional Regulator CTCF Found Essential in Immune Response

Jackie Werner Biomedical Sciences, Hot Topics in Research

CTCF orchestrates the germinal centre transcriptional program and prevents premature plasma cell differentiation Pérez-Garcí­a A, Marina-Zárate E, Álvarez-Prado ÁF, Ligos JM, Galjart N, Ramiro AR. CTCF orchestrates the germinal centre transcriptional program and prevents premature plasma cell differentiation. Nature Communications. 2017;8:16067. http://dx.doi.org/10.1038/ncomms16067. In germinal centres (GC) mature B cells undergo intense proliferation and immunoglobulin gene modification before they differentiate into memory B cells or long-lived plasma cells (PC). GC B-cell-to-PC transition involves a major transcriptional switch that promotes a halt in cell proliferation and the production of secreted immunoglobulins. Here we show that the CCCTC-binding factor (CTCF) is required for the…